Recombinant human ROCK1 protein produced in Sf9 insect cells and supplied with an N-terminal 8xHis tag. Supplied as a 10 μg aliquot in buffered solution (50 mM HEPES, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5), the protein is intended for biochemical assays, activity studies, and antibody validation. It is shipped on dry ice and should be stored at -80°C; avoid repeated freeze-thaw cycles.
- Recombinant human ROCK1 kinase expressed in Sf9 insect cells.
- N-terminal 8xHis tag facilitates purification and detection.
- Purity greater than 90% as determined by reducing SDS-PAGE.
- Approximate molecular weight 50 kDa.
- Supplied in 50 mM HEPES, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5.
- Aliquoted as 10 μg for small-scale assays and validation experiments.
- Shipped on dry ice; store at -80°C and avoid repeated freeze-thaw cycles.